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The influence of epitope availability on atomic-force microscope studies of antigen-antibody interactions.

机译:表位可用性对原子力显微镜研究抗原-抗体相互作用的影响。

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摘要

The ability of the atomic-force microscope (AFM) to detect interaction forces between individual biological molecules has recently been demonstrated. In this study, force measurements have been obtained between AFM probes functionalized with the beta-subunit of human chorionic gonadotrophin (betahCG) and surfaces functionalized with anti-betahCG antibody. A comparison of the obtained results with previous anti-ferritin antibody-binding data identifies differences when the antigen molecule expresses only a single epitope (betahCG), rather than multiple epitopes (ferritin), for the monoclonal antibodies employed. Specifically, the probability of observing probe-sample adhesion is found to be higher when the antigen expresses multiple epitopes. However, the periodic force observed in the adhesive-force distribution, due to the rupture of single antigen-antibody interactions, is found to be larger and more clearly observed for the mono-epitopic system. Hence, these findings indicate the potential of the AFM to distinguish between multivalent and monovalent antibody-antigen interactions, and demonstrate the influence of the number of expressed epitopes upon such binding studies.
机译:最近已经证明了原子力显微镜(AFM)检测单个生物分子之间相互作用力的能力。在这项研究中,已经获得了用人绒毛膜促性腺激素(betahCG)的β亚基功能化的AFM探针与用抗βhCG抗体功能化的表面之间的力测量结果。将获得的结果与以前的抗铁蛋白抗体结合数据进行比较,可以确定当抗原分子仅表达单个表位(βhCG)而不是多个表位(铁蛋白)时的差异。具体地,当抗原表达多个表位时,发现观察到探针-样品粘附的可能性更高。然而,由于单抗原-抗体相互作用的破裂,在粘附力分布中观察到的周期性力被发现更大,并且对于单表位系统更清楚地观察到。因此,这些发现表明了AFM在区分多价和单价抗体-抗原相互作用中的潜力,并证明了表达的表位数目对这种结合研究的影响。

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